Projections of suitable habitat for rare species under global warming scenarios

? Premise of the study: Modeling the contemporary and future climate niche for rare plants is a major hurdle in conservation, yet such projections are necessary to prevent extinctions that may result from climate change. ? Methods: We used recently developed spline climatic models and modified Random Forests statistical procedures to predict suitable habitats of three rare, endangered spruces of Mexico and a spruce of the southwestern USA. We used three general circulation models and two sets of carbon emission scenarios (optimistic and pessimistic) for future climates. ? Key results: Our procedures predicted present occurrence perfectly. For the decades 2030, 2060, and 2090, the ranges of all taxa progressively decreased, to the point of transient disappearance for one species in the decade 2060 but reappearance in 2090. Contrary to intuition, habitat did not develop to the north for any of the Mexican taxa; rather, climate niches for two taxa re-materialized several hundred kilometers southward in the Trans-Mexican Volcanic Belt. The climate niche for a third Mexican taxon shrank drastically, and its two mitotypes responded differently, one of the first demonstrations of the importance of intraspecific genetic variation in climate niches. The climate niche of the U.S. species shrank northward and upward in elevation. ? Conclusion: The results are important for conservation of these species and are of general significance for conservation by assisted colonization. We conclude that our procedures for producing models and projecting the climate niches of Mexican spruces provide a way for handling other rare plants, which constitute the great bulk of the world's endangered and most vulnerable flora.     

Vegetative propagation of Quercus suber L. by somatic embryogenesis. II. Plant regeneration from selected cork oak trees

The regeneration of somatic seedlings from selected 100-year-old cork oak trees is reported. The induction of somatic embryogenesis from leaves of epicormic shoots was significantly affected by genotype, harvesting time and their interaction. Leaves from all five selected trees produced somatic embryos when the segments of branches used as sources of epicormic shoots were collected in May. Genotype, but not the level of photosynthetically active radiation, affected the proliferation of the embryogenic lines and the number of detachable embryos that could be obtained from them. Genotype also affected several steps leading to conversion of somatic embryos, from germination to complete acclimatisation of somatic seedlings. Almost 40% of the somatic embryos from all lines germinated, showing coordinated root and shoot growth. Although the mean percentage of recovery for the whole process was low, plants could be regenerated from four of the five trees tested.     

Genetic transformation of selected mature cork oak (Quercus suber L.) trees

A transformation system for selected mature cork oak (Quercus suber L.) trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses were inoculated with A. tumefaciens strains EHA105, LBA4404 or AGL1 harbouring the plasmid pBINUbiGUSint [carrying the neomycin phosphotransferase II (nptII) and -glucuronidase (uidA) genes]. The highest transformation efficiency (4%) was obtained when freshly isolated explants were inoculated with A. tumefaciens strain AGL1. Evidence of stable transgene integration was obtained by PCR for the nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos were germinated and successfully transferred to soil.Abbreviations BA N⁶-Benzyladenine - GUS -Glucuronidase - MSSH Expression-proliferation medium - NAA -Naphthaleneacetic acid - nptII Neomycin phosphotransferase gene - uidA -Glucuronidase gene     

Influence of 50 Hz electromagnetic fields on recurrent embryogenesis and germination of cork oak somatic embryos

Plant tissue culture techniques are carried out under environmentally controlled conditions in phytotrons. However, electric components of phytotrons generate electromagnetic fields that may act as a environmental factor influencing plant growth and morphogenesis. Isolated somatic embryos of Quercus suber, picked from embryogenic lines, were chronically exposed to a 50 Hz and 15 μT electromagnetic field generated in a Helmholtz-coil system for 8 weeks, in order to examine if the extremely low frequency (ELF) magnetic field (MF) affected the morphogenic behaviour of embryogenic cultures during recurrent embryogenesis. Germination of somatic embryos from genotype G7.1 was carried out under the same electromagnetic field, and also under conditions in which the local geomagnetic field was suppressed. The ELF MF did not influence the growth of embryogenic clumps of the assayed genotypes, but reduced the number of detachable embryos produced by genotype G3.27. The ELF MF did not modify the percentages of germination or plant formation of somatic embryos. However, somatic embryos had better germination when cultured under the suppressed geomagnetic field condition. This revised version was published online in June 2006 with corrections to the Cover Date.     

Identification of metalliferous ecotypes of Cistus ladanifer L. using RAPD markers

The genetic diversity of Cistus ladanifer ssp. ladanifer (Cistaceae) growing on ultramafic and non-ultramafic (basic and schists) soils in the NE of Portugal was studied in order to identify molecular markers that could distinguish the metal-tolerant ecotypes of this species. Random Amplified Polymorphic DNA (RAPD) markers were used in order to estimate genetic variation and differences between populations. The RAPD dataset was analysed by means of a cluster analysis and an analysis of molecular variance (AMOVA). Our results indicate a significant partitioning of molecular variance between ultramafic and non-ultramafic populations of Cistus ladanifer, although the highest percentage of this variance was found at the intra-population level. Mantel's test showed no relationship between inter-population genetic and geographic distances. A series of RAPD bands that could be related to heavy metal tolerance were observed. The identification of such markers will enable the use of Cistus ladanifer in phytoremediation procedures.     

Effect of food restriction on the insulin signalling pathway in rat skeletal muscle and adipose tissue

We tried to elucidate the effects of a brief and severe model of food restriction on insulin sensitivity in female rats, focusing on key proteins involved in the insulin signalling pathway in skeletal muscle and adipose tissue after 5, 10 and 15 days of food restriction. Using euglycemic clamp, we detected that food-restricted rats are significantly less sensitive to insulin action than control rats. However, the time of restriction promotes a progressive increase on insulin sensitivity. The analysis of the insulin signalling pathway showed a tissue-specific regulation of several proteins involved in insulin signalling. In skeletal muscle, insulin receptor substrate 1 and Glut4 are up-regulated at the end of the food restriction period, just the opposite of what we found in adipose tissue. In conclusion, a 50% reduction of food intake modulates insulin sensitivity through a tissue-specific regulation of the insulin signalling pathway in the main target tissues for this hormone.     

A Bootstrap-Based Covariate Selection Method for Modeling the Risk of Lightning-Induced Fires at a Local Scale: A Case Study in Northwest Spain

In lightning-induced fire risk prediction models, the number of potential predictors is usually high, with some redundancy among them. It is therefore important to select the best subset of predictors that obtain models with the greatest discrimination capacity. With this aim in mind, the logistic generalized linear model was used to estimate lightning-induced fire occurrence using a case study of the province of León (northwest Spain). A bootstrap-based test was used to obtain the optimal number of predictors and to model this optimal number of predictors displaying the largest area under the receiver operating characteristics curve. The results show that of the 16 variables initially considered, only three were necessary to obtain the model with the best discriminatory capacity for estimating lightning-induced fire occurrence. Moreover, this model can be considered equivalent to another nine alternative models with three covariates. Both the optimal and the equivalent models are useful in the spatially explicit assessment of fire risk, the planning and coordination of regional efforts to identify areas at greatest risk, and the design of long-term wildfire management strategies. The methodology used for this case study can be applied to other wildfire risk assessment situations where multiple and interconnected covariates are available.     

Antioxidant properties of catechins and proanthocyanidins: Effect of polymerisation,galloylation and glycosylation

A range of catechins and oligomeric procyanidins was purified by high performance liquid chromatography (HPLC) from grape seed, apple skin, lentil and almond flesh. Catechins, galloylated epicatechin, glycosylated catechin, procyanidin dimers, galloylated dimers, trimer, and tetramer species were all identified, purified and quantified by HPLC, LC-MS and NMR. The antioxidant properties of these compounds were assessed using two methods: (a) inhibition of ascorbate/iron-induced peroxidation of phosphatidylcholine liposomes; (b) scavenging of the radical cation of 2,2′-azinobis(3-ethyl-benzothiazoline-6-sulphonate) (ABTS) relative to the water-soluble vitamin E analogue Trolox C (expressed as Trolox C equivalent antioxidant capacity, TEAC). Antioxidant activity in the lipid phase decreased with polymerisation in contrast with antioxidant action in the aqueous phase which increased from monomer to trimer and then decreased from trimer to tetramer. Galloylation of catechin and dimeric procyanidins decreased lipid phase and increased aqueous phase antioxidant activity. Glycosylation of catechin demonstrated decreased activity in both phases.     

Determining Noise in an Aggregates Plant Using Functional Statistics

A key issue to be taken into account in any mining operation or premises is workers’ health and safety. Noise has negative repercussions on workers’ health, which is why regular inspections of noise levels are necessary to ensure that noise is less than legally permitted limits. In our study we statistically analyzed functional data with a view to determining noise levels in an aggregates plant. Data were captured by a sound level meter that indicated equivalent sound pressure levels and peaks and the octave bands for a specific range of frequencies. Unlike the methods habitually used to construct noise maps (which only permit one of these parameters to be determined for each point), the methods applied in our research enable the noise level to be calculated at each point for the entire spectrum of frequencies picked up by a sound level meter. For each point we obtain, rather than a single value, a noise curve reflecting the entire range of frequencies detected by the sound level meter. This enables us to better understand the effects of noise and to determine its origin.